Important information for cell cultivation in the laboratory

The successful cultivation and storage of cell cultures in the laboratory depends on many factors, which we would like to present to you. Ensure the quality of your work by creating steady conditions for your sensitive samples throughout the cultivation process. In addition to a hygienic working method, the choice of your laboratory equipment plays a major role in a working environment. Use modern and reliable technology like chromatographic refrigerators and neutralization systems. We at tritec® are pleased to be providing you with a dependable basis through our high-quality, GMP-qualified laboratory equipment in the field of refrigeration technology and HVAC technology.

Cell cultures and requirements

In the cell culture laboratory, you work with various cultures that require different conditions for optimal growth. A primary culture is derived from organs, tissues or cells obtained directly from an organism. Their cell line reproduces indefinitely, but is conditioned by the natural aging process of the original organism. In contrast, immortalized cells are characterized by their immortality due to unlimited cell division, which may be the result, for example, of a viral infection. A cell strain is spoken of when the cells derived from a primary culture or cell line possess specific properties. This can be achieved by selection or cloning. Adherent cells can only be cultured if they can attach to specific culture vessels. Non-adherent cells, so-called suspension cultures, can be plant, animal or human cells and proliferate in a liquid culture medium, without attachment. Plant cells can also be cultivated in vitro, but with their photosynthetic activity they require an adequate illumination intensity and a suitable light spectrum in order to grow.

Laboratory equipment and supplies

The way you design your laboratory environment is central to optimizing workflows and ensuring the best results. Place your laboratory equipment such as laboratory refrigeratorslaboratory deep freezers and climatic cabinets wisely. For example, biological safety benches should be located in close proximity to CO2 incubators, so that you can quickly (un-)load them. Make sure that your workstation is not exposed to direct sunlight or strong air circulation and that all equipment is aligned with a spirit level. You should also always comply with the manufacturer’s specifications for wall distance.

Use the various culture vessels and nutrient carriers to provide an optimal growth environment for your sensitive cells.

Optimal parameters ensure successful cultivation

In addition to reliable laboratory equipment and comprehensive cell culture knowledge, the following parameters determine the success of cultivation: pH, CO2 concentration, O2 concentration, temperature, and humidity.

Working hygienically at all times is of the utmost importance

Ensuring a consistently hygienic work process is central to the cultivation of cells in a laboratory. This includes your personal work hygiene, exemplary disinfection of the workplace, laboratory equipment and supplies, the use of sterile reagents and media, and conscientious handling to avoid accidental contamination. The structure of your incubator plays a role in this regard as well, because certain design features reduce the risk of contamination.

You should attach particular importance to your own hygiene. After all, people are the biggest potential source of contamination during cell cultivation in the laboratory. Wash your hands regularly, wear protective clothing, and refrain from using cell phones, wrist watches, and rings while working. In addition, when opening an incubator, make sure you know in advance where the sample to be taken is located. This will shorten the time the device is open and also reduce the potential for contamination.

Clean your workplace at least once a day with 70 percent ethanol, remove contaminants (for example spills) immediately and sterilize the incubators conscientiously. Cleaning other laboratory equipment, such as medical refrigerators or blood plasma freezers, as well as your supplies, is equally important. When working with your equipment and media, ensure safe and hygienic handling so that there is no risk of contamination.

With cooling incubators with CO2 fumigation from tritec®, you can rely on additional protection thanks to a design that effectively counteracts contamination. Rounded corners, no internal welding seams and a minimalistic internal design are worth highlighting in this regard. Our spark resistant refrigerator offers further protection in laboratory work. It protects your samples and goods from fire and possible explosions. However, the main focus lies on the constant temperatures that are guaranteed in all our devices. Explore our website and see for yourself.